Background: Diagnosing imprinting defects in neonates and young children presents challenges, often necessitating molecular analysis for a conclusive diagnosis. The isolation of genetic material from oral swabs becomes crucial, especially in settings where blood sample collection is impractical or for vulnerable populations like newborns, who possess limited blood volumes and are often too fragile for invasive procedures. Oral swab samples emerge as an excellent source of DNA, effectively overcoming obstacles associated with rare diseases. Methods: In our study, we specifically addressed the determination of the quality and quantity of DNA extracted from oral swab samples using NaCl procedures. Results: We compared these results with extractions performed using a commercial kit. Subsequently, the obtained material underwent MS–HRM analysis for loci associated with imprinting diseases such as Prader–Willi and Angelman syndromes. Conclusions: Our study emphasizes the significance of oral swab samples as a reliable source for obtaining DNA for MS–HRM analysis. NaCl extraction stands out as a practical and cost-effective method for genetic studies, contributing to a molecular diagnosis that proves particularly beneficial for patients facing delays in characterization, ultimately influencing their treatment. Full article

Toxoplasma gondii is an intracellular parasite that is important in medicine and veterinary science and undergoes distinct developmental transitions in its intermediate and definitive hosts. The switch between stages of T. gondii is meticulously regulated by a variety of factors. Previous studies have explored the role of the microrchidia (MORC) protein complex as a transcriptional suppressor of sexual commitment. By utilizing immunoprecipitation and mass spectrometry, constituents of this protein complex have been identified, including MORC, Histone Deacetylase 3 (HDAC3), and several ApiAP2 transcription factors. Conditional knockout of MORC or inhibition of HDAC3 results in upregulation of a set of genes associated with schizogony and sexual stages in T. gondii tachyzoites. Here, our focus extends to two primary ApiAP2s (AP2XII-1 and AP2XI-2), demonstrating their significant impact on the fitness of asexual tachyzoites and their target genes. Notably, the targeted disruption of AP2XII-1 and AP2XI-2 resulted in a profound alteration in merozoite-specific genes targeted by the MORC–HDAC3 complex. Additionally, considerable overlap was observed in downstream gene profiles between AP2XII-1 and AP2XI-2, with AP2XII-1 specifically binding to a subset of ApiAP2 transcription factors, including AP2XI-2. These findings reveal an intricate cascade of ApiAP2 regulatory networks involved in T. gondii schizogony development, orchestrated by AP2XII-1 and AP2XI-2. This study provides valuable insights into the transcriptional regulation of T. gondii growth and development, shedding light on the intricate life cycle of this parasitic pathogen. Full article

Two surveys were conducted to assess the prevalence and risk factors of ovine caseous lymphadenitis (CL) and explore the association between its superficial and visceral forms in Sardi sheep in Settat province, Morocco. A total of 1521 sheep, including 318 lambs < 6 months, 572 young sheep aged 6–24 months, and 631 adult sheep > 24 months, were examined from 57 flocks. Superficial CL prevalence levels were as follows: 69/318 in lambs, 292/572 in young sheep, and 114/631 in adult sheep. Additionally, 2124 sheep, including 1813 young and 311 adult sheep, were inspected in slaughterhouses ante- and post-mortem. Among all infected animals, the prevalence of visceral abscesses was notably higher in adult sheep (83%, OR = 9.8, 95% CI = 5.5–17.2) compared to young sheep (35%). Data on flock size, sex, age, body condition score, rearing methods, and hygienic measures were collected. Suspected CL cases were confirmed using quadruplex-PCR. Poor barn ventilation, lack of abscess control, and younger age were identified as key risk factors for CL. Additionally, a high percentage (89%) of animals with thoracic abscesses did not display superficial lesions. While age and abscess control have been reported in previous studies, barn ventilation appears to be a new, but important, risk factor. In conclusion, the identified risk factors in Settat province are linked to breeding management practices. Implementing regular abscess control measures and improving barn ventilation are essential for CL prevention. Full article

This work is focused on a novel, promising low temperature phase change material (PCM), based on the eutectic Glauber’s salt composition. To allow phase transition within the refrigeration range of temperatures of +5 °C to +12 °C, combined with a high repeatability of melting–freezing processes, and minimized subcooling, the application of three variants of sodium carboxymethyl cellulose (Na-CMC) with distinct molecular weights (700,000, 250,000, and 90,000) is considered. The primary objective is to optimize the stabilization of this eutectic PCM formulation, while maintaining the desired enthalpy level. Preparation methods are refined to ensure repeatability in mixing components, thereby optimizing performance and stability. Additionally, the influence of Na-CMC molecular weight on stabilization is examined through differential scanning calorimetry (DSC), T-history, and rheology tests. The PCM formulation of interest builds upon prior research in which borax, ammonium chloride, and potassium chloride were used as additives to sodium sulfate decahydrate (Glauber’s salt), prioritizing environmentally responsible materials. The results reveal that CMC with molecular weights of 250 kg/mol and 90 kg/mol effectively stabilize the PCM without phase separation issues, slowing crystallization kinetics. Conversely, CMC of 700 kg/mol proved ineffective due to the disruption of gel formation at its low gel point, hindering higher concentrations. Calculations of ionic concentration indicate higher Na ion content in PCM stabilized with 90 kg/mol CMC, suggesting increased ionic interactions and gel strength. A tradeoff is discovered between the faster crystallization in lower molecular weight CMC and the higher concentration required, which increases the amount of inert material that does not participate in the phase transition. After thermal cycling, the best formulation had a latent heat of 130 J/g with no supercooling, demonstrating excellent performance. This work advances PCM’s reliability as a thermal energy storage solution for diverse applications and highlights the complex relationship between Na-CMC molecular weight and PCM stabilization. Full article

This review summarizes the structural characteristics and physicochemical properties of chiral 4f and 3d-4f complexes based on enantiopure salen-type Schiff base ligands. The chirality originates from the enantiopure diamines and is imparted to the Schiff base ligands and complexes and finally to the crystal structures. The reported enantiopure Schiff base ligands derive from the condensation of aromatic aldehydes, such as salicylaldehyde and its various derivatives, and the enantiopure diamines, (1R,2R) or (1S,2S)-1,2-diamino-cyclohexane, (1R,2R) or (1S,2S)-1,2-diamino-1,2-diphenylethane, (R) or (S)-2,2′-diamino-1,1′-binaphthalene, and 1,2-diaminopropane. Full article

The successful substitution of complex physiological fluids, such as human saliva, remains a major challenge in drug development. Although there are a large number of saliva substitutes on the market, their efficacy is often inadequate due to short residence time in the mouth, unpleasant mouthfeel, or insufficient protection of the teeth. Therefore, systems need to be identified that mimic the functions of saliva, in particular the salivary mucin MUC5B and the unique physiological properties of saliva. To this end, plant extracts known to contain hydrocolloid polysaccharides and to have mucus-forming properties were studied to evaluate their suitability as saliva substitutes. The aqueous plant extracts of Calendula officinalis, Fucus sp. thalli, and lichenan from Lichen islandicus were examined for composition using a range of techniques, including GC-MS, NMR, SEC, assessment of pH, osmolality, buffering capacity, viscoelasticity, viscoelastic interactions with human saliva, hydrocolloid network formation, and in vitro cell adhesion. For this purpose, a physiologically adapted adhesive test was developed using human buccal epithelial cells. The results show that lichenan is the most promising candidate to mimic the properties of MUC5B. By adjusting the pH, osmolality, and buffering capacity with K₂HPO₄, it was shown that lichenan exhibited high cell adhesion, with a maximum detachment force that was comparable to that of unstimulated whole mouth saliva. Full article

This paper proposes an improved method to calculate the mutual capacitance between interdigital transducer (IDT) electrodes to enhance the accuracy of the traditional coupling-of-modes (COM) model, which is commonly used to simulate surface acoustic wave (SAW) filters and duplexers. In this method, the boundary element method (BEM) is adopted to obtain the capacitance per unit length in a layered medium, while the partial capacitance (PC) method is used to derive the effective relative permittivity of the multi-layered IDT. Numerical results from commercially available software are provided for comparison with the results calculated using the proposed method. The consistent results verify the validity and accuracy of this method, which also demonstrates significantly faster calculation speed compared to commercially available software. Precise electrical response prediction of a dual-mode SAW (DMS) filter can be achieved by applying this method to the COM model, and this ultra-fast calculation method can also be included in filter design optimization. Full article

Purpose: To assess the diagnostic performance of contrast-enhanced ultrasound (CEUS) alongside contrast-enhanced computed tomography (CECT) in evaluating central lung cancer (CLC). Materials and Methods: From 2006 to 2022, 54 patients with CLC and obstructive atelectasis (OAT) underwent standardized examinations using CEUS in addition to CECT. The ability to differentiate CLC from atelectatic tissue in CECT and CEUS was categorized as distinguishable or indistinguishable. In CEUS, in distinguishable cases, the order of enhancement (time to enhancement) (OE; categorized as either an early pulmonary arterial [PA] pattern or a delayed bronchial arterial [BA] pattern of enhancement), the extent of enhancement (EE; marked or reduced), the homogeneity of enhancement (HE; homogeneous or inhomogeneous), and the decrease in enhancement (DE; rapid washout [<120 s] or late washout [≥120 s]) were evaluated. Results: The additional use of CEUS improved the diagnostic capability of CECT from 75.9% to 92.6% in differentiating a CLC from atelectatic tissue. The majority of CLC cases exhibited a BA pattern of enhancement (89.6%), an isoechoic reduced enhancement (91.7%), and a homogeneous enhancement (91.7%). Rapid DE was observed in 79.2% of cases. Conclusions: In cases of suspected CLC with obstructive atelectasis, the application of CEUS can be helpful in differentiating tumor from atelectatic tissue and in evaluating CLC. Full article

Background: Guizhou Province in Southwest China has experimented with a centralized hospitalization (CH) treatment for active and severe cases of pulmonary tuberculosis (PTB). The objective of this study was to compare treatment outcomes of patients with tuberculosis (TB) receiving care in a CH setting with those receiving home-based (HB) care. In addition, this study aimed to assess the probability of their household contacts contracting tuberculosis infection. Method: A retrospective review of medical records was undertaken for patients with TB who completed their treatment in four counties in Guizhou, China, spanning from January 2022 to August 2023. In addition, a cross-sectional survey was conducted on the tuberculin skin test (TST) among household contacts of new patients with TB who had completed their treatment. Results: In the retrospective study, 94.8% had successful CH treatment, and 93.1% had successful HB treatment (p value = 0.70). In the prospective study, 559 and 448 household contacts of patients receiving CH treatment had 16 positive and 89 negative TST results, whereas those with HB treatment showed 26 positive and 74 negative TST results. Regarding a logistic regression analysis, the CH group was nearly two times more likely to test negative on the TST, 1.95 (95% CI: 0.98, 3.92). After adjusting for confounding variables, the odds ratio increased significantly to 4.42 (95% CI: 1.22, 16.04). Conclusions: CH for treatment of TB did not show superior success rates, but it may reduce the risk of transmitting tuberculosis infection to household contacts compared to home treatment. Full article

Alginate oligosaccharides (AOS), products of alginate degradation by endotype alginate lyases, possess favorable biological activities and have broad applications. Although many have been reported, alginate lyases with homogeneous AOS products and secretory production by an engineered host are scarce. Herein, the alginate lyase AlyC7 from Vibrio sp. C42 was characterized as a trisaccharide-producing lyase exhibiting high activity and broad substrate specificity. With PelB as the signal peptide and 500 mM glycine as the additive, the extracellular production of AlyC7 in Escherichia coli reached 1122.8 U/mL after 27 h cultivation in Luria-Bertani medium. The yield of trisaccharides from sodium alginate degradation by the produced AlyC7 reached 758.6 mg/g, with a purity of 85.1%. The prepared AOS at 20 μg/mL increased the root length of lettuce, tomato, wheat, and maize by 27.5%, 25.7%, 9.7%, and 11.1%, respectively. This study establishes a robust foundation for the industrial and agricultural applications of AlyC7. Full article

One of the major challenges in designing a wireless indoor–outdoor communication network operating in tunnels and long corridors is to identify the optimal location of the outside station for attaining a proper coverage. It is required to formulate a combined model, describing the propagation along the tunnel and the resulting diffracted outdoor pattern from its exit. An integrated model enables estimations of the radiation patterns at the rectangular tunnel exit, as well as in the free space outside of the tunnel. The tunnel propagation model is based on a ray-tracing image model, while the free-space diffraction model is based on applying the far-field Fraunhofer diffraction equation. The model predictions of sensing the radiation intensity at the tunnel end and at a plane located at a distance ahead were compared with experimental data obtained using a down-scaled tunnel model and shorter radiation wavelength correspondingly. This down-scaling enabled detailed measurements of the radiation patterns at the tunnel exit and at the far field. The experimental measurements for the scaled tunnel case fit the theoretical model predictions. The presented model accurately described the multi-path effects emerging from inside the tunnel and the resulting outdoor diffracted pattern at a distance from the tunnel exit. Full article

This paper addresses the problem of weak maneuvering target detection in the space-based bistatic radar system through long-time coherent integration (LTCI). The space-based bistatic radar is vulnerable to the high-order range migration (RM) and Doppler frequency migration (DFM), since the target, the receiver and the transmitter all can play fast movement independently. To correct high- order RM and DFM, this usually involves joint high-dimensional parameter searching, incurring a large computational burden. In our previous work, a dual-scale (DS) decomposition of motion parameters was proposed, in which the optimal GRFT is conditionally decoupled into two cascade procedures called the modified generalized inverse Fourier transform (GIFT) and generalized Fourier transform (GFT), resulting in the DS-GRFT detector. However, even if the DS-GRFT detector preserves the superior performance and dramatically decreases the complexity, high-dimensional searching is still required. In this paper, by analyzing the structure of the DS-GRFT detector, we further designed a conditioned cubic phase function (CCPF) tailored to the range–slow-time signal after GIFT, breaking the joint high-dimensional searching into independent one-dimensional searching. Then, by connecting the proposed CCPF with the GIFT, we achieved a new LTCI detector called the DS-GIFT-CCPF detector, which obtained a significant computational cost reduction with acceptable performance loss, as demonstrated in numerical experiments. Full article

The TAM receptor ligand Gas6 is known for regulating inflammatory and immune pathways in various organs including the brain. Gas6 becomes fully functional through the post-translational modification of multiple glutamic acid residues into γ-carboxyglutamic in a vitamin K-dependent manner. However, the significance of this mechanism in the brain is not known. We report here the endogenous expression of multiple components of the vitamin K cycle within the mouse brain at various ages as well as in distinct brain glial cells. The brain expression of all genes was increased in the postnatal ages, mirroring their profiles in the liver. In microglia, the proinflammatory agent lipopolysaccharide caused the downregulation of all key vitamin K cycle genes. A secreted Gas6 protein was detected in the medium of both mouse cerebellar slices and brain glial cell cultures. Furthermore, the endogenous Gas6 γ-carboxylation level was abolished through incubation with the vitamin K antagonist warfarin and could be restored through co-incubation with vitamin K1. Finally, the γ-carboxylation level of the Gas6 protein within the brains of warfarin-treated rats was found to be significantly reduced ex vivo compared to the control brains. In conclusion, we demonstrated for the first time the existence of a functional vitamin K cycle within rodent brains, which regulates the functional modification of endogenous brain Gas6. These results indicate that vitamin K is an important nutrient for the brain. Furthermore, the measurement of vitamin K-dependent Gas6 functionality could be an indicator of homeostatic or disease mechanisms in the brain, such as in neurological disorders where Gas6/TAM signalling is impaired. Full article

In the context of strong electromagnetic interference environments, the measurement accuracy of the capacitance parameters of transmission lines under power frequency measurement methods is not high. In this paper, a capacitance parameter anti-interference measurement method for transmission lines based on harmonic components is proposed to overcome the impact of power frequency interference. When applying this method, it is first necessary to open-circuit the end of the line under test. Subsequently, apply voltage to the head end of the tested line through a step-up transformer. Due to the saturation of the transformer during no-load conditions, a large number of harmonics are generated, primarily third harmonic. The third harmonic components of voltage and current on the tested transmission line are extracted using the Fourier transform. The proposed method addresses the influence of line distribution effects by establishing a distributed parameter model for long-distance transmission lines. The relevant transmission matrix for the zero-sequence distributed parameters is obtained by combining Laplace transform and similarity transform to solve the transmission line equations. Using synchronous measurement data from the third harmonic components of voltage and current at both ends of the transmission line, combined with the transmission matrix, this method accurately measures the zero-sequence capacitance parameters. The PSCAD/EMTDC simulation results and field test outcomes have demonstrated the feasibility and accuracy of the proposed method for measuring line capacitance parameters under strong electromagnetic interference. Full article

In the finite element analysis of asphalt concrete (AC), it is nowadays common to incorporate the information from the underlying scales to study the overall response of this material. Heterogeneity observed at the asphalt mixture scale is analyzed in this paper. Reliable finite element analysis (FEA) of asphalt concrete comprises a set of complex issues. The two main aspects of the asphalt concrete FEA discussed in this study are: (1) digital reconstruction of the asphalt pavement microstructure using processing of the high-quality images; and (2) FEA of the asphalt concrete idealized samples accounting for the viscoelastic material model. Reconstruction of the asphalt concrete microstructure is performed using a sequence of image processing operations (binarization, removing holes, filtering, segmentation and boundaries detection). Geometry of the inclusions (aggregate) are additionally simplified in a controlled mode to reduce the numerical cost of the analysis. As is demonstrated in the study, the introduced geometry simplifications are justified. Computational cost reduction exceeds of several orders of magnitude additional modeling error occurring due to the applied simplification technique. Viscoelastic finite element analysis of the AC identified microstructure is performed using the Burgers material model. The analysis algorithm is briefly described with a particular focus on the computational efficiency aspects. In order to illustrate the proposed approach, a set of 2D problems is solved. Numerical results confirm both the effectiveness of the self-developed code and the applicability of the Burgers model to the analyzed class of AC analysis problems. Further research directions are also described to highlight the potential benefits of the developed approach to numerical modeling of asphalt concrete. Full article

Online consumer credit services play a vital role in the contemporary consumer market. To foster their sustainable development, it is essential to establish and strengthen the relevant risk management mechanism. This study proposes an intelligent management framework called the consumer default risk portrait (CDRP) to mitigate the default risks associated with online consumer loans. The CDRP framework combines traditional credit information and Internet platform data to depict the portrait of consumer default risks. It consists of four modules: addressing data imbalances, establishing relationships between user characteristics and the default risk, analyzing the influence of different variables on default, and ultimately presenting personalized consumer profiles. Empirical findings reveal that “Repayment Periods”, “Loan Amount”, and “Debt to Income Type” emerge as the three variables with the most significant impact on default. “Re-payment Periods” and “Debt to Income Type” demonstrate a positive correlation with default probability, while a lower “Loan Amount” corresponds to a higher likelihood of default. Additionally, our verification highlights that the significance of variables varies across different samples, thereby presenting a personalized portrait from a single sample. In conclusion, the proposed framework provides valuable suggestions and insights for financial institutions and Internet platform managers to improve the market environment of online consumer credit services. Full article

Predicting urban morphology based on local attributes is an important issue in urban science research. The deep generative models represented by generative adversarial network (GAN) models have achieved impressive results in this area. However, in such methods, the urban morphology is assumed to follow a specific probability distribution and be able to directly approximate the distribution via GAN models, which is not a realistic strategy. As demonstrated by the score-based model, a better strategy is to learn the gradient of the probability distribution and implicitly approximate the distribution. Therefore, in this paper, an urban morphology prediction method based on the conditional diffusion model is proposed. Implementing this approach results in the decomposition of the attribute-based urban morphology prediction task into two subproblems: estimating the gradient of the conditional distribution, and gradient-based sampling. During the training stage, the gradient of the conditional distribution is approximated by using a conditional diffusion model to predict the noise added to the original urban morphology. In the generation stage, the corresponding conditional distribution is parameterized based on the noise predicted by the conditional diffusion model, and the final prediction result is generated through iterative sampling. The experimental results showed that compared with GAN-based methods, our method demonstrated improvements of 5.5%, 5.9%, and 13.2% in the metrics of low-level pixel features, shallow structural features, and deep structural features, respectively. Full article

When studying the coupling coordination relationship between agricultural eco-efficiency and urbanization, it is crucial to consider food security, especially in a populous country like China. This paper focuses on 31 provinces in China as the research units, covering the time period from 2000 to 2020. Based on the concept of agricultural eco-efficiency, an evaluation index system was developed to include undesirable outputs (carbon emissions), and agricultural eco-efficiency scores were calculated using the SBM–DEA model. An urbanization evaluation index system, covering six dimensions and twelve indexes, was constructed. A comprehensive index of urbanization is measured using the entropy method. On this basis, a coupling coordination model was applied to quantify the relationship between agricultural eco-efficiency and urbanization at the provincial scale in China. The results showed that the agricultural eco-efficiency of all provincial units in China exhibited an overall trend of improvement. Average efficiency followed a spatial pattern of majority grain-consuming areas > grain production–consumption balance areas > majority grain-producing areas. The level of coupling between agricultural eco-efficiency and urbanization is generally low. Currently, no regions have reached the stage of synergy or high-level coupling. Most regions are currently in an antagonistic stage with a coupling degree of 0.3 < C ≤ 0.5. The classification of coupling coordination levels changed from four levels of “severe imbalance”, “moderate imbalance”, “mild imbalance”, and “primary coordination” to “moderate imbalance”, “mild imbalance”, “primary coordination”, and “intermediate coordination”. The level of “severe imbalance” disappeared, the level of “intermediate coordination” appeared, and the level of “mild imbalance” became the largest scale level. From the perspective of food security, the proportion of grain production in the categories of “primary coordination” and “intermediate coordination” was less than 10%, and these provinces never achieved self-sufficiency in food production. The proportion of grain production at the “mild imbalance” level reached 62.4%, while the per capita grain production at the “moderate imbalance” level reached 846.7 kg. Provinces with lower levels of coupling coordination have stronger food security capabilities. It can be observed that the weaker the coupling coordination between agricultural eco-efficiency and urbanization, the higher the food self-sufficiency. Based on the research results above, we discussed strategies to enhance agricultural eco-efficiency in majority grain-producing regions by focusing on technological progress and technical efficiency. Additionally, we analyzed approaches to achieve grain self-sufficiency in regions characterized by a high level of coordination between agricultural eco-efficiency and urbanization, considering both production and trade dimensions. Full article

During the twenty-first century, engineered nanomaterials (ENMs) have attracted rising interest, globally revolutionizing all industrial sectors. The expanding world population and the implementation of new global policies are increasingly pushing society toward a bioeconomy, focused on fostering the adoption of bio-based nanomaterials that are functional, cost-effective, and potentially secure to be implied in different areas, the medical field included. This research was focused on silica nanoparticles (SiO₂-NPs) of bio-based and synthetic origin. SiO₂-NPs are composed of silicon dioxide, the most abundant compound on Earth. Due to their characteristics and biocompatibility, they are widely used in many applications, including the food industry, synthetic processes, medical diagnosis, and drug delivery. Using zebrafish embryos as in vivo models, we evaluated the effects of amorphous silica bio-based NPs from rice husk (SiO₂-RHSK NPs) compared to commercial hydrophilic fumed silica NPs (SiO₂-Aerosil200). We evaluated the outcomes of embryo exposure to both nanoparticles (NPs) at the histochemical and molecular levels to assess their safety profile, including developmental toxicity, neurotoxicity, and pro-inflammatory potential. The results showed differences between the two silica NPs, highlighting that bio-based SiO₂-RHSK NPs do not significantly affect neutrophils, macrophages, or other innate immune system cells. Full article

Mosquito saliva plays a crucial physiological role in both sugar and blood feeding by helping sugar digestion and exerting antihemostatic functions. During meal acquisition, mosquitoes are exposed to the internalization of external microbes. Since mosquitoes reingest significant amounts of saliva during feeding, we hypothesized that salivary antimicrobial components may participate in the protection of mouthparts, the crop, and the gut by inhibiting bacterial growth. To identify novel potential antimicrobials from mosquito saliva, we selected 11 candidates from Anopheles coluzzii salivary transcriptomic datasets and obtained them either using a cell-free transcription/translation expression system or, when feasible, via chemical synthesis. Hyp6.2 and hyp13, which were predicted to be produced as propeptides and cleaved in shorter mature forms, showed the most interesting results in bacterial growth inhibition assays. Hyp6.2 (putative mature form, 35 amino acid residues) significantly inhibited the growth of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Serratia marcescens) bacteria. Hyp13 (short form, 19 amino acid residues) dose-dependently inhibited E. coli and S. marcescens growth, inducing membrane disruption in both Gram-positive and Gram-negative bacteria as indicated with scanning electron microscopy. In conclusion, we identified two A. coluzzii salivary peptides inhibiting Gram-positive and Gram-negative bacteria growth and possibly contributing to the protection of mosquito mouthparts and digestive tracts from microbial infection during and/or after feeding. Full article

Bisphenol A (BPA) and high-fat diets (HFD) are known to adversely affect the kidneys. However, the combined effects of both cases on kidney health and the potential benefits of N-acetylcysteine (NAC) in mitigating these effects have not been investigated. To explore these aspects, male Wistar rats were fed with HFD and allocated to receive a vehicle or BPA. At week twelve, the BPA-exposed rats were subdivided to receive a vehicle or NAC along with BPA until week sixteen. Rats fed HFD and exposed to BPA showed renal dysfunction and structural abnormalities, oxidative stress, inflammation, and mitochondrial dysfunction, with alterations in key proteins related to mitochondrial oxidative phosphorylation (OXPHOS), bioenergetics, oxidative balance, dynamics, apoptosis, and inflammation. Treatment with NAC for 4 weeks significantly improved these conditions. The findings suggest that NAC is beneficial in protecting renal deterioration brought on by prolonged exposure to BPA in combination with HFD, and modulation of sirtuin 3 (SIRT3) signaling by NAC appears to play a key role in the preservation of homeostasis and integrity within the mitochondria by enhancing OXPHOS activity, maintaining redox balance, and reducing inflammation. This study provides valuable insights into potential therapeutic strategies for preserving kidney health in the face of environmental and dietary challenges. Full article

The production of multivariate time-series data facilitates the continuous monitoring of production assets. The modelling approach of multivariate time series can reveal the ways in which parameters evolve as well as the influences amongst themselves. These data can be used in tandem with artificial intelligence methods to create insight on the condition of production equipment, hence potentially increasing the sustainability of existing manufacturing and production systems, by optimizing resource utilization, waste, and production downtime. In this context, a predictive maintenance method is proposed based on the combination of LSTM-Autoencoders and a Transformer encoder in order to enable the forecasting of asset failures through spatial and temporal time series. These neural networks are implemented into a software prototype. The dataset used for training and testing the models is derived from a metal processing industry case study. Ultimately, the goal is to train a remaining useful life (RUL) estimation model. Full article