LDN GmbH

• Booth: 5211

Since its foundation in 1996 LDN has been devoted to the development, production and supply of speciality in-vitro diagnostics test systems and innovative technologies to the clinical and research laboratory markets. The product focus is on immunoassays for Biogenic Amines, Neuroscience, Food Safety and Endocrinology. LDN products are distributed worldwide by a network of OEM partners and distributors and in North America through our daughter company Rocky Mountain Diagnostics.

 Press Releases

• Chromogranin A ELISA (Apr 30, 2024)
  Chromogranin A (CgA) is an acid glycoprotein with 439 amino acids that is present in the secretory dense core granules of most neuroendocrine cells. The chromogranin family consists of at least three different water-soluble acidic glycoproteins (CgA, CgB, and secretogranin II, sometimes called Chromogranin C).
  Upon stimulation, CgA and other peptide hormones and neuropeptides are released. CgA is also secreted from neuroendocrine-derived tumors.
  Neuroendocrine tumors (NETs), which originate from neuroendocrine cells, are found widely distributed throughout the body. The most common sites of NET are the lung, stomach, appendix, cecum, duodenum, pancreas, jejunum/ileum, colon and rectum. NET arising from the gastrointestinal tract are collectively known as gastroenteropancreatic neuroendocrine tumors (GEP-NET) and account for approximately 2/3 of incident NET. The annual incidence of NET is estimated as 2­­­ – 5 cases per 100,000 population.
  CgA is widely expressed throughout the neuroendocrine system and serves as a general biomarker for a wide variety of neuroendocrine tumors. The determination of Chromogranin A helps in the detection of neuroendocrine tumors and is used to assess the course of cancer treatment.
  LDN has developed a two-site sandwich ELISA for the quantitative determination of Chromogranin A (CgA) with the
  following features:
  → Speed and ease of use
  → Suitable for serum samples
  → Standardization with human CgA (5 standards)
  → All reagents ready to use
  → Two controls included
  → Sandwich methodology leads to high specificity
  → Fast: results within 2.5 hours
  → Broad dynamic standard range
  → No high-dose hook effect
  → Easy to automate on open platforms (e.g. Gemini)

• Histamine ELISA (Apr 30, 2024)
  Unique Solutions for Histamine Testing
  Histamine belongs to the biogenic amines and is synthesized by decarboxylation from the amino acid histidine. It is synthesized by mast cells, basophils, platelets, histaminergic neurons, and enterochromaffine cells, where it is stored intracellularly in vesicles and released on stimulation.
  Histamine acts by binding to its 4 receptors (H1R, H2R, H3R and H4R) on target cells in various tissues. It causes smooth muscle cell contraction, vasodilatation, increased vascular permeability and mucus secretion, tachycardia, alterations of blood pressure, and arrhythmias.
  In humans, histamine is one of the most important mediators and takes part in the initial phase of an anaphylactic reaction (“immediate type” allergy).
  
  LDN has developed various ELISAs for a wide range of applications:

  • HISTAMINE in urine, plasma
  • HISTAMINE multi species (for various biological fluids)
  • Histamine Release
  • Histamine in Food
  • Histamine in Fish

  
   

• Serotonin ELISA (Jul 11, 2024)
  Serotonin ELISA - Research and Clinical
  Serotonin (5-hydroxytryptamine) is an intermediate product of tryptophan metabolism, a well-studied neurotransmitter, and may also act as a peripheral hormone. Synthesis occurs mainly in enterochromaffin cells (ec-cells) of the gastrointestinal tract and in neurons. It is present in high concentrations in ec-cells of the intestine, serotonergic neurons of the brain, and platelets. Serotonin is mainly degraded to 5-hydroxyindole acetic acid (5-HIAA) or melatonin and can be excreted in the urine. In the bloodstream, the vast majority of serotonin is found in platelets and can be readily detected in serum.
  Altered serotonin levels in serum and/or urine can indicate both physical and psychological dysfunction.
  Serotonin balance may be impaired in serum and/or urine in a variety of conditions. For example, decreased serotonin levels have been demonstrated in depression, anxiety, and even pain sensitivity compared to unaffected subjects. Increased serotonin levels, on the other hand, have been reported in patients with serotonin-secreting neuroendocrine tumors, also called carcinoid tumors, or hepatocellular carcinomas. 
  LDN offers both - an ELISA for clinical and research applications.