Orochem

340 Shuman Blvd.
Naperville,  IL  60563

United States
https://www.orochem.com

• Booth: 1555

Since 1996, Orochem Technologies Inc. manufactures unique sample prep products, HPLC and UHPLC columns for clinical diagnostic labs in the areas of drugs of abuse, steroids, vitamin D, genomics and proteomics. We also provide related lab process devices. Main Products: Phospholipid Depletion Kits, Polymeric and silica based SPE Plates, and SLLE plates; Protein assay kits, desalting plates, detergent removal resins, affinity purification resins and kits; HPLC columns; Positive Pressure Processor .

 Press Releases

• Rising star: Panthera Deluxe and Panthera Deluxe Pro SPE products (Jul 25, 2024)
  The Panthera Deluxe sorbent is based on poly-DVB and provides both hydrophobic and hydrophilic retention. It demonstrates high capacity and retention for a full range of neutral, basic, and acidic compounds and is particularly suitable for pain management drugs and other pharmaceuticals.
  Panthera Deluxe Pro is the newly upgraded poly-DVB reversed-phase sorbent. It has a narrower particle size distribution and faster flow rate than the Panthera Deluxe. Based on our customers' feedback, its performance beats most of our competitors' products.
  We provide both products and Agility Deluxe ion-exchange products in cartridges, 96-well plates, 96-well microelute plates, and 384-well plate formats.
   

• Orochem's New Desalting Plates Offer Superior Efficiency for Proteomics and (Jul 25, 2024)

  Orochem Technologies is proud to announce the release of two groundbreaking products designed to streamline sample preparation for mass spectrometry analysis: the CORAL 384-Well Protein Desalting Plate and the 384-Well Peptide Desalting Plate.

  

  CORAL 384-Well Protein Desalting Plate

  This innovative high-throughput product is tailored for removing salts and buffers from protein samples, a critical step before mass spectrometry analysis. The CORAL 384-Well Protein Desalting Plate utilizes a size exclusion resin, offering superior desalting efficiency and protein recovery. Key features include:

  • Provisional Patent: The plate incorporates a size exclusion resin under a provisional patent.

  • High Recovery and Efficiency: Achieves over 85% protein recovery and more than 99% desalting efficiency.

  • High-Throughput Capability: Handles 384 samples simultaneously, making it ideal for large-scale studies.

  The general protocol involves simple centrifugation steps to remove storage buffers, equilibrate the resin, and process the samples. The product's catalog number is OCPDSSP384.

  384-Well Peptide Desalting Plate

  Specifically designed for peptide clean-up, the 384-Well Peptide Desalting Plate offers an efficient solution for preparing peptide samples for mass spectrometry. This product features a proprietary resin that effectively desalts both hydrophilic and hydrophobic peptides. Key advantages include:

  • Provisional Patent: Incorporates a unique proprietary resin under a provisional patent.

  • Versatility: Effective for a wide range of peptide types.

  • High-Throughput Format: Processes 384 samples at once, significantly enhancing laboratory throughput.

  The general protocol involves activation of the resin, equilibration, sample loading, washing, and elution, all facilitated by centrifugation. The product's catalog number is OCPPDSP384.

  For further details on these innovative products and how they can benefit your laboratory's mass spectrometry workflows, please visit our product pages or contact us directly:

  • Phone: 630 210 8300

  • Email: info@orochem.com

  About Orochem Technologies Inc.

  Orochem Technologies Inc. is a leading provider of high-quality products for the life sciences industry, specializing in chromatography, sample preparation, and proteomics solutions. With a commitment to innovation and excellence, Orochem aims to empower researchers with tools that drive scientific discovery and advancement.

• Orochem's Universal Filter Plate for Cell Lysates Clarification (Jul 25, 2024)

  Orochem Technologies is proud to highlight our Universal Filter Plate, a cutting-edge solution designed for the efficient and reproducible clarification of cell lysates from bacterial, insect, and human sources. This versatile apparatus supports high-throughput filtration in 24-well, 48-well, and 96-well plate formats, as well as cartridges, transforming the process of cell debris and contaminant removal before the isolation of target molecules like proteins.

  

   

  Key Features:

  • High-throughput capability: Available in 96-well plate format and small-scale single-use depth filtration systems for larger sample volumes (up to 12 mL).

  • Efficiency: Offers rapid processing, reducing the clarification time to just 5 minutes while ensuring high protein and sample volume recovery.

  • Superior results: Provides outcomes comparable or superior to high-speed centrifugation, enhancing the downstream processing of particulate-free feed.

  Procedure Overview:

  • Perform lysate clarification at room temperature or at 4°C.

  • Place the filter plate on top of a collection plate and apply up to 800 µL of cell lysate to each well.

  • Use a variable-speed bench-top centrifuge with appropriate carriers, balancing the assembly before centrifuging at 1000 x g for 2 minutes to collect clarified cell lysate.

  The apparatus features an open-pore structure filter to remove cells and cell debris, while a tighter pore structure filter-aid material simultaneously removes colloidal matter. This dual-action filtering system ensures high-quality clarification, making it an essential tool for researchers and laboratories.

  Experimental Data:

  Research conducted by the Protein Expression Laboratory at the Frederick National Laboratory underscores the effectiveness of Orochem’s Cell Lysate Filter Plate. Comparative SDS Page results demonstrated significant improvement in lysate clarification using the filtration method compared to traditional centrifugation.

  To learn more about Orochem's Universal Filter Plate and its applications, please visit this link.

  For further information and purchasing inquiries, contact us:

  • Phone: 630 210 8300

  • ​​​Email: info@orochem.com

• Orochem's New ZARA OPC Cartridges/Plates: Purify with Precision, Excel in R (Jul 25, 2024)

  Orochem Technologies is excited to introduce our latest innovation in the purification of oligonucleotides—ZARA OPC Cartridges and Plates. These polymer-based, reversed-phase products have a pore size of approximately 300 Å, making them exceptionally stable for organic and basic samples compared to traditional C8 or C18 products.

  

  Key Features of ZARA OPC Products:

  • Versatile Application: Ideal for general oligo purification or DMT-on oligo purification.

  • Effective Impurity Removal: Eliminates salts and traces organic impurities from crude oligonucleotides, providing pure oligonucleotides suitable for sequencing primers, PCR primers, hybridization probes, and gene constructions.

  • High Purity Output: Capable of purifying a wide range of oligos with different molecular weights, from 15 mers to 80 mers.

  • Efficient Separation: Effectively separates DMT-on oligos from other impurities.

  General Protocol for Purification of Oligos:

  1. Flow Rate: Regulate the flow rate of solvents through the cartridge at 1-2 drops/sec.

  2. Sample Preparation: Use amino incubation solution of synthesized CPGs.

  3. Conditioning: Condition the OPC product with 2 x 0.5 mL of methanol, followed by 0.5 mL of acetonitrile, then 2 x 0.5 mL 2.0 M TEAA.

  4. Loading: Load the deprotection mixture (0.1 - 0.3 mL, NH4OH or AMA mixture).

  5. Washing: Wash the OPC with 2 x 1 mL of ammonium hydroxide(1:20), followed by 1 mL of DI water and 0.4 mL of 2% (v/v) acetonitrile in water.

  6. Elution: Elute oligonucleotide from OPC with 0.2 mL of 30% ACN/water. Collect the eluted fraction.

  General Protocol for Purification of DMT-on Oligo:

  1. Flow Rate: Regulate the flow rate of solvents through the cartridge at 1-2 drops/sec.

  2. Sample Preparation: Use amino incubation solution of synthesized CPGs.

  3. Conditioning: Condition the OPC cartridge with 2 x 0.5 mL of methanol, followed by 0.5 mL of acetonitrile, then 2 x 0.5 mL 2.0 M TEAA.

  4. Loading: Load the ammonium incubation mixture (0.1 mL, NH4OH or AMA mixture).

  5. Washing: Wash the OPC with 2 x 1 mL of ammonium hydroxide(1:20), followed by 1 mL of DI water.

  6. Detritylation: Detritylate support-bound oligonucleotide with 2 x 0.5 mL of 2% TFA. A faint pink/orange band may be observed in the cartridge.

  7. Final Wash and Elution: Wash the OPC with 2 x 0.5 mL of water and elute the oligonucleotide from OPC with 0.2 mL of 30% ACN/water. Collect the eluted fraction.

  For more information and to learn more about our ZARA OPC Cartridges/Plates, please downloader our flyer or visit our website at www.orochem.com
  
  Contact us directly:

  • Phone: 630 210 8300

  • ​​​Email: info@orochem.com

• Orochem Technologies Enhances Laboratory Efficiency with Proven High-Throug (Jul 25, 2024)

  Orochem Technologies continues to support laboratory excellence with its trusted high-throughput lab process automation solutions, featuring advanced vacuum manifolds and positive pressure processors. These established tools are designed to enhance laboratory productivity and precision across various applications.

  

  Vacuum Manifold Solutions:

  Orochem’s vacuum manifolds provide reliable and efficient processing for up to 12, 16, or 24 samples simultaneously. Key features include:

  • Individual Vacuum Control: Manage each column independently with replaceable valves.

  • Transparent Glass Chamber: Facilitates clear observation of the sample collection process.

  • Zero Cross Contamination: Disposable needles ensure no risk of sample contamination.

  • Customizable Collection Racks: Accommodate different collection vessels and can be tailored to meet specific needs.

  Our 96-well plate vacuum manifold is ideal for high-volume diagnostics and is compatible with various fiber blocks and liquid handling systems, making it a versatile choice for large-scale laboratory operations.

  Positive Pressure Processors:

   

  The EZYPRESS HT 96 and EZYPRESS 48 Positive Pressure Processors offer reliable performance for solid phase extraction (SPE) applications. Key features include:

  • Modular Rack Design: Easily switch between column sizes and elution racks.

  • Uniform Gas Distribution: Provides consistent pressure and liquid flow across all SPE tubes.

  • Versatile Gas Options: Use inert gases like Nitrogen or Argon for optimal processing conditions.

  The EZYPRESS HT 96 is well-suited for complex samples, delivering precise pressure levels for all steps of SPE. The EZYPRESS 48 caters to smaller batches with the same high level of accuracy and adaptability.

  Orochem Technologies is dedicated to providing reliable solutions that enhance laboratory efficiency and ensure precise results. For more information on our vacuum manifolds and positive pressure processors, please visit:

  • Vacuum Manifold Solutions

  • Positive Pressure Processor Solutions

  For additional inquiries, please contact us directly:

  • Email: info@orochem.com 

  • Phone: 630-210-8300