Hzymes Biotechnology Co., Ltd.

• Booth: 4745

Established in 2015,WUHAN HZYMES BIOTECHNOLOGY CO.,LTD.is a high-tech enterprise specializing in biomedicine, R&D,production and application of core materials of in vitro diagnosis,and technical services. It has five R&D and production centers. Hzymes technical platforms： enzyme mining & characterization, enzyme molecule optimization &modification,high-throughput screening of enzyme genes, organic synthesis, synthetic biology, and antigen-antibody R&D and production.

 Press Releases

• About Hzymes Biotech (May 09, 2025)
  Hzymes Biotechnology Co., Ltd., founded in 2015, is a high-tech enterprise specializing in R&D, production, application, and technical services for core raw materials for biomedicine and in vitro diagnostics. The company maintains an extensive business network with marketing and R&D centers in Shanghai, Wuhan, and Yichang.
  
  
  Technologically, the company has established specialized platforms including enzyme gene mining & characterization, enzyme molecular engineering, high-throughput screening, organic synthesis, synthetic biology, and antigen/antibody development. Leveraging these platforms, it has developed hundreds of core raw materials for biomedicine, vaccine production, and in vitro diagnostics.
  
  Hzymes Biotech operates a pharmaceutical-grade specialty enzyme production system featuring ultra-large-scale capacity and ultra-high equipment standards in manufacturing. Currently serving over 2,400 biomedicine, vaccine, and IVD reagent manufacturers worldwide, its products are exported to more than 40 countries and regions. The company collaborates closely with academic institutions such as Shanghai Jiao Tong University, Shanghai Institute of Laboratory Medicine, and Suzhou Institute of Biomedical Engineering and Technology (CAS), integrating cutting-edge life science technologies to accelerate the commercialization of research achievements.
  
  Guided by its mission to "innovate foundational technologies to benefit life" and core values of "Client-Centric Approach, Innovation, Excellence, Accountability, and Win-Win Collaboration," Hzymes Biotech continues to expand its product portfolio and advance toward its vision of becoming "a global leader in core biotechnologies."
   

• Advanced Insights into Hzymes’ PCR/RT-PCR CDMO Services (May 09, 2025)

  Hzymes PCR/RT-PCR Service Portfolio

  Hzymes Biotech is recognized for its expert team, extensive project experience, and commitment to client satisfaction. The company has successfully delivered numerous customized PCR service projects, ensuring high-quality and reliable outcomes.

  1. PCR Primer and Probe Design Optimization

  Precise primer and probe design are critical to PCR performance. Hzymes offers tailored optimization services, ensuring high specificity, sensitivity, and reproducibility.

  2. PCR Reagent Performance Optimization

  Hzymes optimizes reagent formulations to enhance stability, sensitivity, and consistency, providing customized solutions to meet client-specific requirements.

  3. Lyophilization Process Development for PCR Kits

  Lyophilization is essential for creating stable and long-lasting PCR kits. Hzymes offers custom lyophilization solutions, ensuring product integrity and ease of use.

  4. PCR CDMO Service

  As a full-service CDMO, Hzymes provides complete PCR solutions, from process development and scaling to quality-controlled manufacturing, ensuring cost-effective production.

  5. PCR OEM Service

  Hzymes offers OEM (Original Equipment Manufacturer) services, including white-label manufacturing, customized packaging, and branding to help clients launch their PCR products.

  6. PCR Kit Custom Development Service

  Hzymes provides complete custom PCR kit development, ensuring rapid prototyping and tailored designs from concept to final product.

  Why Choose Hzymes Biotech for PCR/RT-PCR Services?

  • Expert PCR Project Team: Highly skilled scientists with in-depth knowledge.
  • Extensive Project Experience: Successful delivery of numerous client projects.
  • Efficient Project Response: Streamlined project management for fast turnaround.
  • Fast Project Delivery: Optimized production processes for rapid delivery.

• Enzyme Preservation Magic – Decoding the Development of Enzyme Lyophiliza (May 09, 2025)

  Challenges of Enzyme Lyophilization: How to Lock in 100% Activity

  Biological products such as proteins, enzymes, and antibodies are prone to physical degradation (e.g., denaturation, aggregation) and chemical reactions (e.g., oxidation, hydrolysis) when in solution, leading to instability in their structure and function [1]. With the rapid development of biotechnology, many biological products have begun to adopt lyophilization technology to extend their shelf life and improve transportation convenience. This method removes moisture from the substance by directly sublimating it from the solid state to the gaseous state, thereby preserving the original biological activity of the sample without damaging its structure, achieving long-term stable storage. However, during the actual lyophilization process, for biological active molecules such as enzymes that are extremely sensitive to environmental conditions, they may be affected by various adverse factors, including low-temperature stress [2], concentration effects [3], changes in pH value [4], phase separation [5], and dehydration stress, causing reversible or irreversible changes in the protein structure, thereby affecting activity. Ensuring the effective retention of enzyme activity before and after lyophilization has become an urgent technical problem that needs to be solved. In addition, even under ideal storage conditions, enzyme preparations still face the risk of gradually losing activity during storage, affecting the product's final effect and increasing production costs.
  
   

  Key Factors Affecting the Activity and Stability of Enzyme Lyophilization

  Key Parameters of Lyophilization Process: Unlocking the Best Path

  Lyophilization mainly includes key stages such as pre-freezing, annealing, primary drying, and secondary drying. Precise control of the conditions and parameters of each step is crucial for ensuring the biological activity and stability of enzyme preparations after lyophilization, among which the freezing temperature in the pre-freezing stage, the collapse temperature and heating rate in the primary drying stage, and the temperature and time in the secondary drying stage are particularly important.

   

• HZYMES’ Creatinine Test Kits: A Comprehensive Innovation in Kidney Health (May 09, 2025)

  Kidney disease is a growing global concern, affecting approximately 850 million people worldwide. In China, chronic kidney disease has reached a prevalence rate of 8.2%, impacting one in every ten people. This makes kidney health a critical issue, underscoring the importance of reliable diagnostic methods. Creatinine levels, a byproduct of muscle metabolism, are one of the most essential biomarkers for assessing kidney function. Elevated creatinine levels often signal impaired kidney function, making accurate and efficient testing methods vital for early detection and management.
  
  The Need for Improved Creatinine Testing Methods

  Traditionally, clinical creatinine testing relied on the Jaffe reaction, also known as the alkaline picrate method. While this method has been widely used, it is prone to interference from other substances, leading to inaccurate results. In contrast, enzymatic creatinine testing, which involves methods such as creatinine amidohydrolase and creatinine oxidase, offers superior specificity, accuracy, and resistance to interference. Among these, the creatinine oxidase method stands out, providing a broader linear range and enhanced reliability, particularly in clinical settings where sample quality can vary.

  However, despite the advances in enzymatic methods, challenges such as reagent stability, interference from substances like calcium dobesilate, and the complexity of multi-enzyme systems remain. This is where HZYMES steps in with a groundbreaking approach to creatinine testing.
  
   

  HZYMES’ Innovations in Creatinine Testing

  HZYMES has taken the enzymatic testing method a step further, introducing key innovations that significantly enhance the performance of creatinine test kits. The company has focused on improving the stability and accuracy of the core enzymes involved in the testing process. Two key enzymes—creatinase and catalase—play pivotal roles in ensuring reliable and consistent results.

  Creatinase and Catalase Upgrades

  Creatinase, a crucial enzyme in the creatinine detection system, hydrolyzes creatinine into intermediate compounds for further analysis. HZYMES has optimized this enzyme through enzyme-directed evolution, resulting in the 13M4 creatinase mutant, which demonstrates enhanced thermal stability and enzyme activity. This innovation has led to a substantial increase in the enzyme’s half-life and performance, ensuring stable reagent functionality even under challenging conditions.

  Another important enzyme, catalase, is added to eliminate interference from endogenous creatine, which can skew test results. HZYMES has made significant strides in optimizing catalase's structure and purification process, achieving a remarkable 20-fold increase in its half-life at elevated temperatures. This upgrade ensures that the reagents maintain their performance over extended periods, even after prolonged exposure to high temperatures.

• Hzymes Biotech’s Integrated Immunochemiluminescence Substrate Solutions (May 09, 2025)

  Hzymes Biotech is a leading innovator in immunology diagnostics, providing high-quality chemiluminescence substrate solutions designed to enhance sensitivity, stability, and precision in immunoassay applications. Our integrated solutions, including APS-5 and AMPPD chemiluminescence substrates and solutions, offer superior performance for accurate enzyme-linked immunoassays (ELISA) and clinical diagnostics.

  APS-5 Chemiluminescence Solutions: Fast and Accurate Detection

  APS-5 Chemiluminescence Substrate (HHCS-1)

  • High Sensitivity: Capable of detecting alkaline phosphatase (ALP) as low as 1×10⁻¹⁴ mol/L (0.01 pg).
  • Fast Peak Intensity: Reaches a stable peak within seconds for rapid analysis.
  • Wide Detection Range: Covers ALP concentrations from 50 to 50,000 pg/mL.
  • Extended Linearity: Ensures seven orders of magnitude in linear detection.
  • Temperature Stability: Operates efficiently between 22-35°C without requiring strict temperature control.

  APS-5 Chemiluminescence Solution (HHCS-4)

  • Offers the same high sensitivity and rapid detection capabilities as HHCS-1.
  • Provides a broad detection range and extended linearity, making it ideal for clinical laboratories.
  • Maintains temperature insensitivity, ensuring consistent results without specialized equipment.

  AMPPD Chemiluminescence Solutions: Long-Term Stability for Reliable Diagnostics

  AMPPD Chemiluminescence Substrate

  • Ultra-Sensitive Detection: ALP detection as low as 1×10⁻¹⁴ mol/L (0.01 pg).
  • Broad Detection Range: Supports linear detection between 50-50,000 pg/mL.
  • Long Shelf Life: Stable for up to 2 years when stored at 2-8°C, away from light.

  AMPPD Chemiluminescence Solution

  • Features the same high sensitivity and detection range as the AMPPD substrate.
  • Ensures extended storage stability, making it a cost-effective choice for diagnostic applications.

  Why Choose Hzymes Biotech Chemiluminescence Solutions?

  • High Sensitivity: Ensures accurate detection even at extremely low concentrations.
  • Rapid Signal Generation: Provides quick and consistent results for fast diagnostics.
  • Broad Detection Range: Supports a wide variety of immunoassay applications.
  • Temperature Stability: Works effectively without precise temperature control.
  • Long Shelf Life: Reduces waste and enhances cost efficiency.