The RM21® MicroMirror TIRF microscope is a unique Single Molecule Microscope. Spatial separation of the excitation and emission beams and the use of broadband micromirrors leads to superior signal-to-noise ratios for multi-wavelength TIRF microscopy. The open access to the excitation and emission optical pathways makes this the ideal platform to implement a range of biophysical techniques simultaneously. Among the available options, Magnetic Tweezer, dichroic TIRF, iSCAT.