SCIENION US, Inc.

Tempe,  AZ 
United States
http://www.scienion.com

• Booth: 1579

SCIENION provides systems and services for the contact-free printing of single cells, biological and chemical agents for diagnostics, pharmaceutics, veterinary, plant and food analytics. Our dispensers allow for precise spotting in the pico- to micro-liter range on a variety of substrates, from silicon wafers and microtiter plates, to lateral flow strips. We now carry the best colorimetric and fluorescence array scanners. New sciPOLY allows immobilization without surface functionalization

 Press Releases

• cellenONE® Unparallel clonal recovery achieved in 96 and 1536 Microtiter Plates (Jul 24, 2019)

  The main challenges associated with cell line development are maintaining high cell viability and ensuring monoclonality of isolated cells. Unlike flow cell sorters, which induce high stress and compromise cell viabilities, automated cloning using cellenONE is a gentle and precise method to achieve unparalled clonal recoveries in both standard (96 MTP) and high density (1536 MTP) substrates. This is demonstrated with CHO cells, one of the most common cell line used in recombinant therapeutic proteins manufacturing.

  The number of wells containing single CHO colonies were counted for each plate (six 96 MTP and one 1536 MTP) and the percent of wells containing single colonies was calculated (Figure 3).

  Outstanding clonal recoveries were observed with 96% and 94% of wells containing single colonies in 96 and 1536 MTP, respectively (Figure 2). Additionally, the remaining wells were empty and did not contain multiple colonies. To our knowledge, this is the first time such high clonal recoveries have been demonstrated with CHO cells in both standard (96 MTP) and very high density (1536 MTP) substrates.

  This experiment demonstrates the cellenONE as an ideal platform for undertaking automated cloning in both standard and high density substrates as it ensures monoclonality while maintaining outstanding clonal recovery.

  Compatibility with high density substrates offers a dramatic increase in throughput to automatically process the equivalent of 16 individual 96 MTP in under an hour leaving personnel free to perform less routine tasks.

  In addition to saving time, one can save on consumables and expensive reagents while ensuring documentation and monoclonality of all isolated cells.https://www.cellenion.com/fileadmin/user_upload/Downloadable_Area_pdfs/Application_Note_cellenONE_Unparallel_clonal_recovery_achieved_in_96_and_1536_MTP.pdf

• Shaping the future of Diagnostics through MULTIPLEXING and SINGLE CELL approach (Jul 24, 2019)

  This year, SCIENION and cellenion will organize a yearly wkrshop in Lyon, from 3rd to 4th of September.

  The agenda will incude two sessions every day, presenting fully automated testing platforms from sample to results and new technologies

  SESSION 1. Fully Automated Testing Platforms From Sample to Results
  09:00 - 9:30 MOBIDIAG
  Novodiag® Diagnostic Platform
  9:30 - 10:00 Master Diagnostica
  Multiplexing Molecular Diagnostic kits based on DNA-Flow Technology
  10:00 - 10:30 MyCartis
  Antelope Dx: Diagnostics where you need it
  10:30 - 10:45 Break
  10:45 - 11:15 Infynity Biomarkers
  Changing the paradigm of serology testing from single markers to
  multiplex testing: Study model of Chagas disease
  11:15 - 11:45 AXO Science
  105plex for the straightforward characterization of bacteria and resistance

  SESSION 2. New Technologies: Trends for Tomorrow
  13:30 - 14:00 Biopalitra
  Multiplexed immunoassays for biomarkers of HBV, HCV, HIV & Treponema
  pallidum
  14:00 - 14:30 Joanneum Research
  High-throughput Roll-to-Roll Production of Biosensors
  14:30 - 15:00 Nanovation
  Nano-Based Sensors for Respiratory Monitoring: From Lab to Market
  15:00 - 15:15 Break
  15:15 - 15:45 University of Lyon
  3D bioprinting and ink-jet bioprinting hybrid method for tissue engineering
  15:45 - 16:15 Génoscope - Sequencing National Center
  Cell fate decisions implicated on brain complexity

  On the second day, the focus will be single cell isolation and its applications.

  SESSION 3. Unravelling Cancer Genomics at Single Cell Level
  09:00 - 9:30 University of British Columbia Cancer Research Center
  Sequencing the DNA present in single sorted cells
  9:30 - 10:00 German Cancer Research Center (DKFZ)
  Epigenetic regulation in stem cells and cancer
  10:00 - 10:30 Centre Léon Bérard
  Single cell analysis in immuno-oncology : R&D, diagnosis,
  prognosis and much more
  10:30 - 10:45 Break
  10:45 - 11:15 Institute of Cancer Research London
  Isolating single-cell clones to measure cancer heterogeneity
  11:15 - 11:45 Biolidics
  Phenotyping characterization of CTC in cancer using spiral
  microfluidic enrichment
  11:45 - 12:15 Gilupi
  In vivo capturing of CTCs – Towards Personalized Medicine in Oncology
  12:15 - 12:30 Q&A
  12:30 - 13:30 Coffee at the Exhibitors´ Room
  
  SESSION 4. Emerging Applications in Single Cell Isolation
  13:30 - 14:00 University of Jena
  Isolating single clones for CRISPR/CAS application and
  spotting beads for single cell analyses
  14:00 - 14:30 University of Rennes
  Microbiota analysis : Towards post-metagenomics and post-
  metatranscriptomics
  14:30 - 15:00 Suzhou Institute of Nano-Tech and Nano-Bionics
  One technology with dual uses: microarray and single cells
  analysis in mAb discovery

  Register here

• Dual Color Multiplex Lateral Flow Assay for Food Intolerance (Jul 24, 2019)

  Food allergies represent an increasingly significant health issue around the world.  Over 160 foods are known to cause allergic reactions in humans.  The U.S. Food & Drug Administration (FDA) has identified 8 food groups that are responsible for 90 percent of clinically defined (IgE-mediated) acute food allergies. 

  In this study, a lateral flow based multiplex immunoassay was developed to measure the levels of IgE (allergy) and IgG (food intolerance) in patients with known food allergies.  Food protein extracts were printed onto nitrocellulose membrane strips in an array format (LFM-lateral flow microarray). Subsequently, undiluted plasma from patients was applied and their food specific IgE and IgG profiles determined in a single conjugate pad. Various LFM formats were evaluated.

  Multiplex  & Miniaturized Lateral Flow Tests for Multianalyte Detection

  Based on traditional production technology, conventional LFAs rely on lines (control and test lines) arranged perpendicular to the flow direction. Since this design has a limitation in multiplex degree and the possibility of depletion and inadvertent interferences of assay components in multiparametric tests, there is a need for advanced array-based lateral flow assay layouts.

  SCIENION technology provides high precision and high reproducibility deposition of low volume into any surface, including nitrocellulose-based membranes. SCIENION dispensing systems, sciFLEXARRAYERs, enable miniaturization and multiplexing of classic lateral flow tests to achieve multianalyte detection.

  Here, we report on the development of LFM for the assessment of food allergy (IgE-mediated) and food intolerance (IgG-related). Food protein extracts were first deposited with sciFLEXARRAYER onto nitrocellulose membrane as an array and subsequently incorporated into a standard LF format for testing.

  Visit our booth #1579 to ge your copy

 Additional Info