Creative Diagnostics is pleased to announce the introduction of its new Pseudotyped GFP HPV, a valuable tool for researchers studying human papillomavirus (HPV).
HPV consists of more than 100 genotypes and can cause cervical cancer and its precursors. Currently, 12 HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59) have been classified as human carcinogens, and an additional 8 types have been classified as possible human carcinogens. Creative Diagnostics can provide up to 15 different types of HPV pseudoviruses, including all 12 oncogenic HPV types.
Our pseudotyped GFP HPV production is based on transfection of the 293 cell line 293FT, which has been modified to express high levels of SV40 large T antigen. The cells are co-transfected with codon-modified papillomavirus pod genes L1 and L2 and a pseudo-genomic plasmid containing the SV40 replication source. Pseudovirus (PsV) encapsidated with a GFP reporter plasmid can be used to develop high-throughput in vitro neutralization assays in a 96-well plate format.
In addition, Creative Diagnostics offers a broad range of replication-incompetent pseudotyped reporter viruses that express surface proteins of various target viruses and contain reporter genes such as luciferase, GFP and RFP.
Advantages
- No need to handle live infectious viruses.
- Reporter genes facilitate the detection and quantification of viral activity.
- Can be used to study virus function and entry, screen antiviral drugs and neutralizing antibodies, and assist in other important experimental procedures.
Features of Reporter Virus Particles
- Safe in a BSL-2 environment
- Quantitative (luciferase) or fluorescent (GFP, RFP) read-out
- For high-throughput antiviral neutralization assays
To learn more about the Pseudotyped GFP HPV product list, please visit the Creative Diagnostics website at https://www.creative-diagnostics.com/news-pseudotyped-gfp-hpv-108.htm.